A novel polymer-mineral ZRO2-GD2O3 radiopaque nanocomposite with hyaluronic acid coating for repair of bone defects in experiment P.78-83
polymer-mineral radiopaque nanocomposites based on
ZrO2-Gd2O3 nanoparticles with polyelectrolyte-hyaluronic
acid shell (Z1 and Z2) to enhance repair of artificially
created defect in caudal vertebra bone of rats.
Material and Methods. 18 white outbred female rats of 8-9
months of age and 300-350 g body weight were used in the
experiments. The animals were divided into 3 groups, each
including 6 rats. An artificial defect was produced in rat's
vertebra and further regeneration of the osseous tissue was
performed by using synthesized nanocomposites Z1 and Z2
of different consistency. ²n the control group, the bone defect
was sutured below the blood clot. Radiological
investigations were carried out in different terms of bone
regeneration (15 and 30 days after surgical intervention).
Results and Discussion. Created biomaterials demonstrated
biocompatibility as soon as in the two first weeks after
surgery. Their application was not accompanied by
inflammatory reaction and suppuration of the regenerate,
opposite to formation of large areas of destruction of the
osseous tissue during regeneration of bone defect in control
group of rats in which regeneration of the defect was
performed under blood clot. High density of Z1 material
allows keeping it in the centre of the bone defect, opposite
to Z2 material leaking from the defect area (15 and 30 days
after surgical intervention).
Conclusions. Novel in vivo experimental model of caudal
vertebra proved its efficiency in testing materials used for
regeneration of the osseous tissue. Created polymer-mineral nanocomposite based on ZrO2-Gd2O3 nanoparticles with the
hyaluronic acid shell effectively enhanced regeneration of
bone defect. The clinical biotolerance, radiopacity, as well
as high integration of applied Z1 material with the osseous
tissue of recipient bed have been demonstrated.
Keywords: ZrO2-Gd2O3, radiopaque nanocomposite, osseous
defect, rat's caudal vertebra
Danilchenko SN, Kalinkevich OV, Pogorelov MV,
Kalinkevich AN, Sklyar AM, Kalinichenko TG, Ilyashenko
VY, Starikov VV, Bumeyster VI, Sikora VZ, Sukhodub LF:
Characterization and in vivo evaluation of chitosanhydroxyapatite
bone scaffolds made by one step
coprecipitation method. Journal of Biomedical Materials
Research 2011, 96A(4), 639-647.
Zhang L, Jiang Y, Ding Y, Povey M, York D: Investigation
into the antibacterial behaviour of suspensions of ZnO
nanoparticles (ZnO nanofluids). Journal of Nanoparticle
Research 2007, 9(3), 479-489.
Kim HD, Valentini RF: Retention and activity of BMP-2 in
hyaluronic acid-based scaffolds in vitro. Journal of
Biomedical Materials Research 2002, 59, 573-584.
Hunt DR, Jovanovic SA,. Wikesjo ME, Wozney JM, Bernard
GW: Hyaluronan supports recombinant human bone
morphgenetic protein-2 induced bone reconstruction of
advanved alveolar ridge defects in dogs. A pilot study.
Journal of Periodontology 2001, 72, 651-658.
Konstantinova T, Danilenko ², Glazunova V, Volkova G,
Gorban O: Mesoscopic phenomena in oxide nanoparticles
systems: processes of growth. Journal of Nanoparticle
Research 2011, 13(9), 4015-4023.
Novikov V, Zaichenko A, Mitina N, Shevchuk O, Raevska
K, Lobaz V, Lubenets V, Lastukhin Y: Inorganic, polymeric
and hybrid colloidal carriers with multi-layer reactive shell.
Macromolecular Sympjsium 2003, 210, 193-202.
Horak D, Shagotova T, Mitina N, Trchova M, Boiko N,
Babic M, Stoika R, Kovarova J, Hevus O, Benes MJ,
Klyuchivska O, Holler P, Zaichenko A: Surface-initiated
polymerization of 2-hydroxyethyl methacrylate from
heterotelechelic oligoperoxide-coated γ-Fe2O3
nanoparticles and their engulfment by mammalian cells.
Chemical Materials 2011, 23, 2637-2649.
Zaichenko A, Voronov S, Kuzaev A, Shevchuk O, Vasiliev
V: Control of microstructure and molecular weight
distribution of carbon-chain heterofunctional
oligoperoxidic curing agents. Journal of Applied Polymer
Science 1998, 70, 2449-2455.
European convention on protection of vertebrate animals,
used in investigation and other scientific aims. Strasbourg,
April, 18, 1996: official translation [Electronic resource]
Verhovna Rada (Supreme Council) of Ukraine. - Official
web site. - (international document of European Council).
- Access mode to the document: http://zakon.rada.gov.ua/
Hodovana OI, Stoika RS, Herashchenko SB, Delcova OI,
Kljuchivska OYu, Zaichenko OS: Technique of experimental
investigation of osseous tissue regeneration in systemic
osteoporosis. Patent of Ukraine 100354, 2012, Dec10.
- Поки немає зовнішніх посилань.
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