A MORPHOMETRIC CHARACTERISTIC OF RATS SPLEEN AT PRE-SLAUGHTER STRESS UNDER THE USE OF BIOLOGICALLY ACTIVE SUBSTANCES P.73-77

S.S. GRABOVSKYI

Анотація


Material and Methods. The spleen extract, the Echinacea and
Chinese lemon extracts (70° alcohol solutions), and the
sprouted grains were added to the diet of the rats of all
experimental groups by aerosol method as antistressors and
immunomodulators in pre-slaughter period (five days before
slaughter). Only 70° alcohol solution in the same volume and
the same method was added to the diet of the rats of the control
group five days before slaughter. The experiment continued for
five days in the vivarium; laboratory animals were taken from
the cage in turn, weighed and decapitated under ether
anaesthesia. Research results were processed statistically using
the software package Statistica 6.0 and Microsoft Excel for
Windows XP. Probability differences were assessed by Student
t-test.
Results and Discussion. Some changes in the morphological
structure of the spleen tissue were observed at pre-slaughter
stress in laboratory animals. The histological investigation of
the rat spleen of the experimental groups established a distinct
fission of the red and white pulp. The red pulp formed the bulk
of the organ's parenchyma and presented as a net of reticular
fibers passing into the collagen fibers, fibers trabeculae and
capsules. Reticular mesh is permeated by venous sinuses, with
erythrocytes, lymphocytes, plasma cells, white blood cells and
other blood components located in the loops. The largest
number of lymphatic nodes with react centers were formed in
laboratory animals additionally fed with spleen extract (I
experimental group); changes of their average area were found,
compared to control.
Conclusions. The results obtained in model experiments on
laboratory animals in the vivarium can be used in research on
agricultural animals to prevent the effect of their pre-slaughter
stress on the quality of meat products.
Key words: rats, spleen, pre-slaughter stress, spleen extract,
echinacea and Chinese lemon extracts, sprouted grains

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