ANALYSIS OF THE RATS' LIVER BINUCLEATE HEPATOCYTES VOLUME CHANGE UNDER THE INFLUENCE OF OPIOIDS DURING THE THREE WEEKS EXPERIMENT P.69-72

S.O. VOVK, M.V. LOGASH, Yu.Ya. KRYVKO

Анотація


Aim. To analyze the morphological changes of the rat liver,
particularly the changes of the volume of binucleate hepatocytes
during the experiment.
Materials and Methods. White male rats, 190-230 gr. of weight,
were selected for the study; two randomized groups of animals
were formed: ² - the control group of rats, ²² - the experimental
group (receiving intramuscular injections of opioid
"Nalbufin"). The dose of the opioid was increased every week.
"Nucleator" method was applied to determine the volume of
hepatocytes.
Results and Discussion. It was determined that the hepatocytes
volume varies in different weeks of the experiment. The median
indices testify that the hepatocytes volume decreases in the 3-
rd week (1697 μm3), while in the 1st and 2nd weeks the cell
volume increases to 3157 μm3 and 3479 μm3 respectively. The
value of standard deviation in the 1st week was close to the
control - 2672, the maximal on the 2nd week - 3833, while the
minimal on the 3rd week - 2221, respectively. The maximal
mean of the volume of the cells was revealed in the 2-nd week
(4539 μm3) of experiment, while the decrease of the mean of the
cell volume occurred in the 3rd week - 2338 μm3.
Conclusion. Thus, according to the data, the results of our
investigation show the dynamics of the volume of the binucleate
hepatocytes during all time periods of the experiment. The
volume of the cells reaches its maximum in 2nd week, and its
minimum - in 3rd week.
Key words: opioids, binucleate hepatocytes, nucleator, liver

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