ANTIMICROBIAL ACTIVITY OF A COMBINED γ-CROTONOLAKTONE AND Zn-CARNOZINE DERIVATIVE GEL IN VITRO AND IN VIVO P.16-21

R.Z. OGONOVSKY

Анотація


Aim:To investigate the antimicrobial activity of a gel mixture
of γ-crotonolaktone and Zn-carnozine derivatives in vitro and
in vivo by examining its effects on healing of experimental
infected dermatome wounds in white rats.
Methods: The method of diffusion in agar was used to check the
antimicrobial activity of the test mixture using standard
organisms, including Staphylococcus aureus (ATCC 25923),
Esherichia coli (ATCC 25922), Pseudomonas aeruginosa
(ATCC 27853), Enterococcus faecalis (6783), Bacillus subtilis
(ATCC 6633), and Candida albicans (ATCC 184). As screening
preparations, Myramistin-Darnytsa, Oflacin-Darnytsa,
Levomekol, 5% Syntomicine emulsion, and 10% methyluracil
ointment were used. An experimental wound was created using
the standard methods of Abdulaiv with the help of a rounded
stencil with a general area of 120 mm2
(diameter 12.5 mm). All animals (white rats weighing 180-220 g) were divided into three groups: control animals that received no treatment; a group in which the surface of the wound was covered with the new 2% mixture gel; and another group in which the surface of the wound was treated with Oflacin-Darnytsa ointment. The wounds were evaluated at 3, 5, 7, 10, 14, and 21 days after they were created. To determine the antimicrobial activity of the
treatments applied, material from the surface of the wound was
removed with a sterile tampon. After 24 hours of incubation,
the number of colonies was counted, and the result was the
number of colony-forming units per 1 nm 2
of wound.
Results: The test mixture showed high antimicrobial potential:
the least sensitivity was seen with cultures of S. aureus, with
inhibition of growth to 20.50±0.22 mm, while the greatest
sensitivity was seen with cultures of B. subtilis (25.33±0.42
mm). The in vitro testing showed that the new mixture was
superior to some modern preparations, such as MyramistinDarnytsa ointment and was practically identical in effectiveness
to Oflacin-Darnytsa ointment. Under in vivo conditions,
Staphylococcuss organisms disappeared after 7.9±0.73 days
with treatment with the new gel, while they disappeared after
9.8±0.63 days with Oflacin-Darnytsa ointment. In the control
group, in which the wounds healed over without external
interference, the microflora from the surface of wound
disappeared only after 12.7±0.48 days.
Conclusions:The positive results seen with this new mixture
suggest the need for further development and clinical research
of new preparations based on this mixture for the treatment of
infectious-mechanical damage of soft tissues and other similar
diseases.

Повний текст:

PDF

Посилання


Beresyiakov A.B. Experimental reperative activity research

of ointment of "Glitsyt" at the aseptic and infected wounds

of skin / A.B. Beresyiakov, S.B. Popov, O.A. Ruban //

Ukrainian biopharmaceutical magazine. - 2010. - №6 (11). -

С. 42-44. Ukrainian: (Березняков А.В. Експериментальне

дослідження репаративної активності мазі "Глітацид" на

асептичні та інфіковані рани шкіри / А.В. Березняков,

С.Б. Попов, О.А. Рубан // Український біофармацевтичний

журнал - 2010. - №6 (11). - С. 42-44.)

Treatment of the infected wounds and wound infection.

Train aid. / F.V. Galimsianov // Ekaterinburg: USMA, 2012.

- 88 с. Russian: (Галимзянов Ф.В. Лечение инфицированных

ран и раневой инфекции. Учебное пособие. /

Ф.В. Галимзянов // Екатеринбург: УГМА, 2012. - 88 с.)

Kon`kov D.G. Pharmatherapeutical efficiency of ointments

which contain vybrion at experimental wounds: aftoref.

dis. on the receipt of sciences degree of kand.med.science:

special.14.03.05 "Pharmacology"/ D.G.Kon`kov. - Odessa,

- 20 p. Ukrainian: (Коньков Д.Г. Фармакотерапевтична

ефективність мазей, що містять відборон, при експериментальних ранах: автореф. дис. на здобуття наук.

ступеня канд.мед.наук: спец.14.03.05 "Фармакологія"/

Д.Г.Коньков. - Одеса, 2009. - 20 с.)

Ogonovsky R.Z. Phase speed of the experimental infected

wound healing on a background an adrenalin damage

myocardium and her correction / R.Z. Ogonovsky, M.S.

Regeda, Yu.B. Pasternak // Achievement of biology and

medicine. - 2012. - № 3. - С. 16-19. Ukrainian: (Огоновський

Р. З. Пофазна швидкість загоєння експериментальної інф

ікованої рани на тлі адреналінового пошкодження міокард

і та її корекції / Р. З. Огоновський, М.С. Регеда, Ю. Б.

Пастернак // Досягнення біології та медицини. - 2012. -

№ 3. - С. 16-19.)

Tsyganenko A. Ja. Etiology of festering-inflammatory

diseases of skin and soft tissues and sensitiveness to the

antibiotics of basic causative agents / A.Ja. Tsyganenko,

E.V.Gyrych, O.A.Golovina // Experimental and clinical

medicine. - 2008. - № 1. - С. 66-68. Russian: (Цыганенко А.

Я. Этиология гнойно-воспалительных заболеваний кожи

и мягких тканей и чувствительность к антибиотикам основных

возбудителей / А. Я. Цыганенок, Е. В. Гирич, О.

А. Головина // Експериментальна і клінічна медицина. -

- № 1. - С. 66-68.)

Shalimov O.O. Modern medicinal treatment of wounds :

department instruction / O.O. Shalimov. - К., 2002. - 35 c.

Ukrainian: (Шалімов О. О. Сучасне медикаментозне лі-

кування ран : відомча інструкція / О. О. Шалімов. - К.,

- 35 c.)

Development of a Bayesian model to estimate health care

outcomes in the severely wounded / A. Stojadinovic, J.

Eberhardt, T. S. Brown [et al.] // J. Multidiscip. Healthc. -

- Vol. 6, № 3. - P. 125-135.

Hindhede A. A clinical case-series evaluation of a

superabsorbent dressing on exuding wounds / A.

Hindhede, F. Meuleneire // Journal of Wound Care. - 2012.

- № 11. - P. 576-580.

Kieser D. C. Leading wound care technology : The ARANZ

medical silhouette / D. C. Kieser, C. Hammond // Adv. Skin

Wound Care. - 2011. - № 2. - P. 68-70.

Lees P. Pharmacokinetics, pharmacodynamics and

therapeutics of pradofloxacin in the dog and cat / P. Lees /

/ Journal of Veterinary Pharmacological Therapy. - 2012. -

№ 12. - P. 111-116.

Nanoengineering a biocompatible inorganic scaffold for

skin wound healing / G. E. Poinern, D. Fawcett, R. K.

Brundavanam [et al.] // J. Biomed. Nanotechnol. - 2010. -

Vol. 6, № 5. - P. 497-510.

Pastar I. Micro-RNAs: New Regulators of Wound Healing

/ I. Pastar, H. Ramirez, O. Stojadinovic and oth. // Surgical

Technology International. - 2011. - №12. - P. 51-60.

Thomson Ch. Yakult: a role in combating multi-drug

resistant Pseudomonas aeruginosa? / Ch. Thomson, I.

Hassan, K.Dunn // Journal of Wound Care. - 2012. - №11. -

P. 568-569.

White R. Wound colonization and infection: the role of

topical antimicrobials/ R. White, R. Cooper, A. Kingsley //

Brіtish Journal Nursery. - 2011. - Vol. 10, № 9. - P. 563-578.


Посилання

  • Поки немає зовнішніх посилань.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.