A STATE OF ALIMENTARY TRACT IMMUNE SYSTEM IN RATS UNDER ANATOMICALLY CHANGED CONDITIONS P.70-75

T. KAMINSKI, D. WANICZEK, E. KAMINSKA, I. RUBEL-SNIETURA

Анотація


Appendix, solitary and intestinal lymph follicles, mucous
lymphocytes make a cluster of gut- associated lymph tissue
called GALT. Alimentary tract lymphocytes provide two
basic tasks; they produce Ig A being the first defence line
against pathogenic micro-organisms and regulate processes
of immunologic response to antigens reaching its lumen. To
recognize GALT structure and its function better, the
experimental study on 21 sexually mature male rats of Wistar
strain was carried out. The animals were divided into 2
study groups A and B each consisting of 7 rats and 1 control
K consisting of 7 rats. The rats from one study group A had
an appendectomy and the rats from the other group B had
ileocolic lymph nodes removed. The rats from the control
group K underwent only laparotomy. After 63 days, the
respective organs were taken and the animals were
sacrificed. The appendices and ileocolic lymph nodes
functioning for 63 days in the anatomically changed
conditions underwent macro- and microscopic examination
and were compared wih the image of the control group
organs. No significant morphologic differences were found.
Additionally, immunohistochemical examination was
carried out with specific antibodies Mous Anti Rat CD 45
RA aimed against all B lymphocytes and Mous Anti Rat CD
43 aimed against all T lymphocytes and the evaluation of
the immunohistochemical reaction intensity was done with
the use of a computer image analyzer. A decreased average
number of B lymphocytes was found in the ileocolic lymph
node parenchyma of the experiment animals; mean number
of T lymphocytes was on the same level. The quantitative
ration of B lymphocytes to T lymphocytes changed. Similar
immunologic changes can be present in other immune system
organs; therefore, it seems necessary to continue the
experiments and study.
Key words: GALT (gut associated lymphoid tissue), immune
system, appendectomy, lymph nodes, lymphology

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Bijnen C.L.,Van Den Broch W.T., Bijnen A.B., De Ruiter P.,

Gouma D.J.: Implication of removing a normal appendix.

Dig. Surg. 2003; 20 (2): 115-121.

Ikinciogullari A., Dogu F., Ikinciogullari A., egin Y., Babacan

E. : Is immune system influenced by adenotonsillectomy

in children? Int. J. Pediatr. Otorinholaryngol. 66 (3) 2002

Dec2 251-257.

Brandtzaeg P.: The human intestinal immune system: basic

cellular and humoral mechanisms. Baillieres Clinical

Rheumatology 1996; 10: 1-24.

MacDonald T.: The raaction of the immune system ton

pathogens but not food antigens and commensial bacteria,

Semin. Immunol. 13 (2001) 159-161.

Komuro T., Hashimoto Y.: Three-dimensional structure of

rat intestinal wall (mucosa and submucosa). Archives of

Histology and Cytology 1990; 53:1-21.

Belisle C., Sainte-Marie G.: The narrowing of high endothelial

venules of the rat lymph node. Anat. Rec.,1985; 211: 184-

Pabst R.: The antomical basis for the immune function of

the gut. Anatomy and Embryology 1987; 176; 135-144.

Slusarczyk K. Marniok B. Poreba G., Kaminski T.:

Wspolczesne poglady na budowe wezla chlonnego.

Chirurgia Polska 2000; 2: 169-173.

Slusarczyk K.: Anatomia drog odplywu chlonki i

regionalnych wezlow chlonnych watroby szczurow szczepu

Wistar i Lewis. Rozprawa Habilitacyjna, Sl.A.M, Katowice

Castenholz A.: Architecture of the lymph node with regard

to its function. Curr. Top. Pathol., 1990; 84: 2-31.

Pospisil R., Mage R.: Rabbit appendix: a site of

development and selection of the B cell repertoire. Curent

Opinions in Microbiology, Immunology 1998; 229: 59-70.

Belise C. Sainte-Marie G.: Tridimensional study of the

deep cortex of the rat lymph node: topography of the deep

cortex. Anat. Rec., 1980; 199: 45- 59.

Belisle C., Sainte-Marie G.: Topography of the deep cortex

of the lymph nodes of various mammalian species. Anat.

Rec., 1981; 201: 553-561.

Andersson R.E., Olaison G., Tysk C., Ekbom A.:

Appendectomy is followed by increased risk of Crohn

disease. Gastroenterology 124 (1) 2003 (Jan) 40-46.

Watson W.S., Hampartzoumian T., Lloyd A.R., Grimm

M.C.:A murine model of appendicitis and the impact of

imflammation on appendiceal lymphocyte constituents.

Clin. Exp. Immunol. 2007 October; 150 (1): 169-178.

Slusarczyk K. Poreba G., Marniok B., Ciszek M., Kaminski

T.: Naczynia o wysokim srodblonku a cykl plciowy.

Chirurgia Polska 2000; 2: 237-241.

Belisle C. Sainte-Marie G.: Tridimensional study of the

deep cortex of the rat lymph node: The deep cortex units of

germ-free rat. Am. J. Pathol., 1982; 107: 70-78

Sainte-Marie G., Peng F.S., Belisle C.: Overall architecrure

and pattern of lymph flow in the rat lymph node. Amer. J.

Anat., 1982; 164: 275-309

Liu N.F., Maldik J., Olszewski W.: Mesenteric lymph node

transplantation in syngenic rats: changes in cellularity and

architecture. Lymphology 1992; 25: 139-144.

Olszewski W.L., Machowski Z., Nielubowicz J.: Badania

limfograficzne po przecieciu naczyn chlonnych u psa. Pol.

Przegl. Chir., 1986; 9: 960-966.

Andreu-Ballester J.C., Perez-Greira J., Colomer-Rubio E.,

Ortiz-Tarin I., Pennarroja-OteroC. : Secretory

immunoglobulin A deficiency in serum of patients with

GALTectomy (appendectomy and tonsillectomy). Clin

Immunol 2007; 123: 289-297.

Andreu-Ballester J.C., Ballester F., Colomer-Rubio E.,

Millan-Scheiding M. Association between tonsillectomz,

adenoidectomy and appendicitis. Rev. Esp. Enferm. Dig.

(3) (2005 Apr) 179-186.


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